This study aims at improving learning outcomes and student motivation of Chemistry Education FKIP ULM through Cooperative Learning Model-based ACSI (Action Cards Speak Independent). This study used experimental research with one-group pretest-posttest design. The population was the student of chemistry education FKIP ULM, and sampling technique is purposive sampling. Data Collection Technique used pretest and post-test paper, motivation questioner, and unstructured interview. Data analysis techniques used N-gain test and motivation analysis criteria. The finding showed that there was improvement in the learning outcomes with good enough categorie analyzed by N-gain test whose score was 0,64 and motivation questioner which resulted score 84,5% showed this study aroused the very good students’ motivation.