Iron (Fe) is a metal that is present in high concentrations in coal dust. Exposure to coal dust could increase the concentration of Fe in the body and can cause several health problems, including liver cells damaged. However, the mechanism of liver cell damage by Fe in the coal dust is still not clear. In this present study, we proposed that Fe could induced liver cells damaged via chlorinative stress pathway. Thus, our study aimed to investigated the effect of Fe on liver cells via chlorinative stress pathway by measuring the levels of hydrogen peroxide (H2O2) and Advanced Oxidation Protein Products (AOPPs), the myeloperoxidase (MPO) acivity, and chlorinative index (CI). The results revealed that Fe exposure could significantly increased H2O2 and AOPPs level, and CI in liver cells (P<0.05). The result also revealed that Fe exposure could decreased the MPO activity but non-statistically significant (P>0.05). These results indicated that Fe exposure induce chlorinative stress which can be seen from the increasing of H2O2 and AOPPs levels, and CI. However, the exact mechanism of Fe induced the chlorinative stress is still unclear yet, because the MPO activity did not statistically changed. Further study may be needed to determine the Fe mechanism to induce chlorinative stress.