In Indonesia, utilization of papaya leaf (Carica papaya Linn.) as a cooked vegetable and traditional herbal medicine (called jamu) is very common. Some of local community consume jamu of papaya leaf daily to keep their body fitness and prevent from some illness. Scientific investigation to deliver comprehensive evidences of the traditional and medicinal uses of papaya leaf is still not adequate, especially in the aspect of its biological active constituents, Antioxidative and vasodilatatory effects of methanol extract of papaya toaf that implicate in the reduction of cardiovascular risks have been demonstrated. This Study was focused on the isolation and identification of the active constituents of methanol extract of C papaya leaf. Variety of C papaya used in this experiment was IPB9 that collected in Peiaihari, South Kalimantan. Chromatographic isolation was performed using several sizes of open columns with two different polarity of stationary phases, silica 60 (0.063-0,200 mm) and ODS (12 nm, S-75 µm), which was preceded by alcoholic and fractionation process. Structures of the isolated compounds were by comparison of their physical (appearance, color, melting point) and spectroscopic data (UV-Vis, IR, 1H- and 13C-NMR) with those reported in the literatures, eight isolated compounds were identified as gallic acid, caffeic acid, mauritianin, myricitrin, kaempferol rutinoside, quercetin, and kaempferol, which belonging to compounds.