In line with the implementation of regional autonomy and decentralization, local government (local government) have an increasingly important role in influencing the performance of the regional economy. Business climate is one of the prerequisites to the implementation of a dynamic economy. Created by government regulation to encourage or otherwise impede the creation of a conducive business climate. Regional Economic Policy Research Business and Investment Climate in Hulu Sungai Tengah is a study to look at linkages, economic conditions, investment climate and a thriving business in the district of Hulu Sungai Tengah that maintains Natural Resources. This study is an attempt to map and analyze the Business Climate and Investment areas related to business and investment in the District of Hulu Sungai Tengah without having to exploition be condition of natural resources. Textually, this study uses aspects of the condition of facilities and infrastructure to support investment and business climate, economic conditions in regions with regional Banua Anam was supported by a discussion of substance, and the principle as a reference in analyzing local problems regulations and the potential impact on the world of business and investment. Regional economic policy in a development without having to destroy and exploit natural resources, but can increase the welfare of the community in a sustainable manner. This study concluded the practice of the implementation of some local regulations in relation to the business and investment climate in the areas of business services, trade in agricultural products and food, and industrial processing and regulation of various sectors of the regional economy. Investment climate in the district of Hulu Sungai Tengah is quite conducive and adequate infrastructure so that investment in this area is quite attractive in sectors that do not exploit the natural resources thus the region's autonomy will be more focus on green GDP that does not rely on the mining sector which can damage environmental sustainability. This is consistent with the vision of regional development mission that emphasize sustainable development. Key words: business climate, investment. Regulation