Abstract:
Galam (Melaleuca cajuputi) is a native peatland species that naturally associated with endophytic
fungi. The endophytic fungi known for its ability as biofungicide. The aims of this research was to
investigate the ability of endophytic fungi isolated from Galam tree to inhibit the growth of
pathogenic fungi C.truncatum and also to analyze the mechanism of the inhibition of the pathogen by
the endophytic fungi. This study included (a) isolation of the endophytic fungi; (b) screening of the
endophytic fungi; (c) antagonistic assay of the endophytic fungi; (d) volatile and nonvolatile
metabolite examination; and (e) microparasitism observation. The result showed that Neurospora sp.
DG 27 exhibited highest antagonistic activitu against C. truncatum by 71,98% of the pathogen growth
inhibition, followed by Neurospora sp. DG 17 with 53.74% and Syncephalastrum sp. AG 15 with
48.28%. Inhibition through volatile and non-volatile metabolites production was showed by
Neurospora sp. DG 27. Observation on mycoparasitism assay showed that Neurospora sp. DG 27
exhibited perform antibiosis mechanism , while DG 17 isolate and Syncephalastrum sp. AG 15
exhibited competition and mycoparasite mechanisms