Uji Antagonisme Kapang Endofit Tanaman Galam (Melaleuca cajuputi) terhadap Colletotrichum truncatum

Abstract

Galam (Melaleuca cajuputi) is a native peatland species that naturally associated with endophytic fungi. The endophytic fungi known for its ability as biofungicide. The aims of this research was to investigate the ability of endophytic fungi isolated from Galam tree to inhibit the growth of pathogenic fungi C.truncatum and also to analyze the mechanism of the inhibition of the pathogen by the endophytic fungi. This study included (a) isolation of the endophytic fungi; (b) screening of the endophytic fungi; (c) antagonistic assay of the endophytic fungi; (d) volatile and nonvolatile metabolite examination; and (e) microparasitism observation. The result showed that Neurospora sp. DG 27 exhibited highest antagonistic activitu against C. truncatum by 71,98% of the pathogen growth inhibition, followed by Neurospora sp. DG 17 with 53.74% and Syncephalastrum sp. AG 15 with 48.28%. Inhibition through volatile and non-volatile metabolites production was showed by Neurospora sp. DG 27. Observation on mycoparasitism assay showed that Neurospora sp. DG 27 exhibited perform antibiosis mechanism , while DG 17 isolate and Syncephalastrum sp. AG 15 exhibited competition and mycoparasite mechanisms