Abstract:
Abstract—Seluang fish (Rasbora spp.) besides contain high protein also contain an essential fatty acids (PUFA), which can increase intelligence. The objective of this research was
to prove that Seluang fish is one of the nutrient source to
increase the spatial memory and expression of PPAR brain
tissue by using Rattus norvegicus as a model. Rats were divided into 3 groups: control group were fed standard (C),
Treatment Group 1 were fed low-protein and fat (P1), Group Treatment 2 (P2) were fed standard with Seluang
Formula, for 8 weeks. The first phase was to testing spatial memory using the Morris Water Maze. The second phase, was the making brain tissue’s slide preparation,
immunohistochemical stained, calculated the percentage of
PPARá and PPARã expression. The data analysed using
ANOVA and Kruskal Wallis test with a 95% confidence level. The first phase results showed the percentage of
period time in the target area for the P2 group is the highest
compared to the C and P1 group, respectively 33.52%; 29.9%; and 23.15% (p = 0.021). On PPARá and PPARã
examination showed the average percentage of PPARá neurons expression in group P2 is higher than the C and P1
group, respectively 18.5%, 3.75% and 2.5% (p = 0.002)
Average percentage of total PPARã neuronan cells
expression in P2 group also higher than the C and P1 group,
respectively 35.41%, 6.25% and 5.83% (p = 0.001). The study shows that rats fed with Seluang Fish Formula able to
improved the brain tissue’s spatial memory and PPAR
expression.
