Hospital is a health care institution which organizes personal health services in the plenary that provides inpatient, outpatient, and emergency department. Medical support officer in a hospital is a human resource which is non-physical infrastructure that is necessary for the achievement of organizational goals. Leadership as human resource which is occupying a key position or the policy makers are no less important for the success of an organization itself. The highest level of leadership or hospital directors is spearheading leadership in directing all available resources, both physical and non-physical so that the organization be able to make changes towards continuous improvement. The purpose of this research is to know the influence of the leadership, work experience and work motivation on performance of nursing employee personnel in Banjarmasin. This research method using the quantitative survey. This research technique using the formula of slovin with a sample of 108. The results showed no influence of leadership with employee performance (p-value= 1.000), there is no influence between work experience with employee performance (p-value= 1.000), there is no influence between motivation and employee performance (p-value= 0.462). The conclusion of this study there was no influence of leadership, work experience, and motivation on performance of nursing employee at hospital in Banjarmasin. Keywords: Leadership, Work Experience, Motivation