Copper wire cable and fiber optic cable are two communication media that are widely used in building data communication networks in today's modern era. For network administrators, choosing the right type of cable to build a network is a must. Air temperature is one of the external factors that can affect the performance of network equipment. This paper provides a comparative analysis of the differences in performance between the use of fiber optic cables and copper wire cables which are capable of transferring data of 1 Gigabit per second. Performance measurement analysis includes the ability to transfer data from both media such as latency, throughput, and packet loss. For testing latency and throughput is done by sending as many as 65,000 data 30 times for each media. Whereas for packet loss testing is done by sending 10,000 data within 1 minute using test bandwidth on the Mikrotik router. From the test results, it can be seen that there is an effect of temperature changes on the performance of copper wire cable and fiber optic cable. The higher the air temperature, the packet loss, and latency will increase. As for the throughput value, the temperature only affects the throughput value on fiber optic cable and does not affect throughput on the copper wire cable. Key words: Copper wire, fiber optic, latency, packet loss, throughput.