Hospital nurses are always faced different kinds of patients’ illness, both infectious and non- infectious. Therefore, nurses should have sufficient knowledge and attitude in using PPE when providing care to patients so that covered from being infected by the disease. Good behavior towards the use of PPE may increase the professionalism of the nurse in front of patients. This study aims to determine the correlation between relationship between knowledge, attitude and the use of PPE. This is a quantitative research using cross sectional approach. Sampling was done by purposive sampling techniques were determined using the criteria for inclusion. Sample in this study were nurses in Surgery Room and Internal Disease Room Panglima Sebaya Hospital with a total sample of 38 people. Research instruments using questionnaires and observation sheets. Data analysis used Fisher exact test with a significance level of 95% (α = 0.05). Based on research, the results show that there is significance correlation between knowledge and the use off PPE (p <0.010), and significance correlation between attitude and the use of PPE (p <0.0001). Keywords: knowledge, attitudes, the use of PPE, nurse