The enamel thickness of gestational diabetes mellitus rat offspring

Abstract

Objective: Gestational Diabetes Mellitus is diagnosed when Diabetes Mellitus begins or is first detected during pregnancy. Enamel hypoplasia is an abnormality in tooth enamel that was common found in children born to diabetic mothers. The aim of this study was to determine the molar enamel thickness of rat offsprings born to diabetic rat. Material and Methods: This research used sixteen rat offsprings born to 8 diabetic rats who had been injected with STZ dose of 40 mg / kg and 16 rat offsprings born to 8 control rats. Rat offsprings were decapitated on day 5 after birth and taken right mandibular molar tooth germ. Tooth germ then embedded in paraffin block. Haematoxylin eosin staining performed for histological analysis. Enamel thickness measured on the images using ImageJ Software at 5 field of view. Results: The enamel thickness on the DM group (333.02 ± 135.81 µm) were lower than control group (415.39 ± 146.17 µm). Mann Whitney test results showed a significant difference between the DM and control group. Conclusion: It was concluded that enamel thickness decrease in gestational diabetes mellitus rat offspring.