Abstract:
Objective: Gestational Diabetes Mellitus is diagnosed when Diabetes
Mellitus begins or is first detected during pregnancy. Enamel hypoplasia
is an abnormality in tooth enamel that was common found in children
born to diabetic mothers. The aim of this study was to determine the
molar enamel thickness of rat offsprings born to diabetic rat.
Material and Methods: This research used sixteen rat offsprings born
to 8 diabetic rats who had been injected with STZ dose of 40 mg / kg and
16 rat offsprings born to 8 control rats. Rat offsprings were decapitated
on day 5 after birth and taken right mandibular molar tooth germ.
Tooth germ then embedded in paraffin block. Haematoxylin eosin
staining performed for histological analysis. Enamel thickness
measured on the images using ImageJ Software at 5 field of view.
Results: The enamel thickness on the DM group (333.02 ± 135.81 µm)
were lower than control group (415.39 ± 146.17 µm). Mann Whitney
test results showed a significant difference between the DM and
control group.
Conclusion: It was concluded that enamel thickness decrease in
gestational diabetes mellitus rat offspring.
