Abstract Gel of Mauli banana stem from South Kalimantan had shown to accelerate wound healing by increasing the number of neovasculars on the 5th day. The study aimed to determine the potencial of gel of Mauli banana stem extract (EBPM) towards increased expression of HIF- 1α, Hsp90α, and VEGF in healing traumatic ulcer. This research was a true experimental with 40 rats of Rattus norvegicus of male Wistar strain as the traumatic ulcer models. The biopsy was taken from the ulcer in the left buccal mucous. The study was divided into the negative control group (K) given gel, treatment Group 1 (P1) given gel of 25% EBPM, treatment group 2 (P2) given gel of 37.5% EBPM, and Treatment Group 3 (P3) given gel of 50% EBPM. On the 3rd and the 5th days, the models were decapitated. They were next biopsied in order to make preparations, and to conduct immunohistochemical test. The results proved that the EBPM gel was effective when administered at a concentration of 37.5% in the 5 th day because it significantly increased the expressions of HIF-1α, Hsp90α, and VEGF. It can be concluded that the EBPM gel within concentration of 37.5% can be recommended as a topical drug for healing traumatic ulcer. Keywords: Musa acuminata stem, HIF-1α, Hsp90α, VEGF, Healing