Abstract:
This study aims to select and obtain antigens of thelocal strain A. hydrophila bacteria in Sungai Batang and Mandiangin villages, South Kalimantan, which are immunogenic to African catfish. Antigen is inactivated using the heating method (heat killed). The design of this study used a completely randomized design (CRD)with 6 treatments and 3 replications which included vaccination with the antigen Aeromonas hydrophila strain P5.1 (A), strain AM01 (B), strain P1.1 (C), strain P3.1 ( D), strain P4.1 (E) and control were injected with PBS pH 7.0 (K). Antigen was injected intramuscularly as much as 0.1 ml (bacterial density 109 cells/ml)per test fish (A, B, C, D, E). Theresults of the antigen immunogenicitytest of local strains P5.1 and P1.1 were more immunogenic to African catfish compared to other antigens based on their ability to increase post-vaccination antibodies. Antigencross-reaction test A. hydrophila local strains AM01, P1.1 and P5.1 are
capable of cross-reacting with other antigens. P1.1 antigen is able to recognize more antigens than P5.1 and AM01, so it can be recommended as a vaccine candidate
