The effectiveness of Dayak onion (Eleutherine palmifolia) extracts for medicinal treatment of Sangkuriang catfish (Clarias gariepinus) infected with different doses of bacteria Aeromonas hydrophilla

Abstract

This research was performed to investigate the effectiveness of Dayak onion (Eleutherine palmifolia) extracts used to treat the Sangkuriang catfish (Clarias gariepinus) experimentally infected with bacteria Aeromonas hydrophilla. Observation and measurement were carried out in the three different laboratories. A total of 150 individuals of catfish were periodically investigated in 15 circle containers. A 22 factorial completely randomized design was applied as the experimental design, comprising 4 treatment combinations (bacteria dose and onion extract) and 3 replications. Treatment A1B1: 1.25106 cfu/mL and 40 ppm, treatment A1B2: 1.25106 cfu/mL and 70 ppm, treatment A2B1: 1.25108 cfu/mL and 40 ppm, and treatment A2B2: 1.25108 cfu/mL and 70 ppm. After 17-day rearing period, the treatment A1B1 provided the best performance for hemoglobin (7.70 g/dL), leucocrit (1.33%), blood plasma (56.67%) and survival rates (86.67%). While the best result for hematocrit level (41.33%) was given by the treatment A2B1. The effective dose of onion extract was fixed at 40 ppm. Water temperature, pH, DO, and NH3 were ranged as follows: 26.5 - 26.9 ºC, 6.8 - 7.0, 6.4 - 6.9 mg/L, and 0.00 - 0.05 mg/L. These levels correspond to the tolerance limits of the catfish. The outcome of this research could be beneficial for better aquaculture practices