Abstract:
Determining the 3D structure of proteins using laboratory instrumentation is time-consuming and
expensive. The in silico method can be used as an alternative to predict the 3D structure of proteins, such
as the fold recognition method. This study aims to create a 3D structural model of rice's (Oryza sativa)
protein superoxide dismutase (SOD). The 3D structure modeling of the protein was carried out with the
Phyre2 web server. The protein sequence was obtained from the UniProt KB database with the code
A0A6F8FUX1. The results showed that the suitable template used to build the model was the template with
the code c1unfX. The c1unfX template has a coverage value of 80%, 100% confidence, and i.d. of 51%.
Validation of the model with the PROCHECK program showed that the most favored area on the
Ramachandran Plot was 87.8%, and the disallowed area was 1.1%. The disallowed area, which is still
below 15%, indicates that the three-dimensional structure model of the SOD protein built from the c1unfX
template has good a value.
Keywords: fold recognition, Phyre2
, 3D protein structure, superoxide dismutase
