Abstract:
Immobilization of enzymes through metal-based
system is demonstrated as a promising approach to enhance
its properties. In this study, the influence of metals ions,
including copper, cobalt and zinc (Zn) on the immobi lization of b-glucosidase (BGL) through the synthesis of
protein-inorganic hybrid was evaluated at 4 C. Among
these metal ions-based hybrids, Zn showed the highest
encapsulation yield and relative activity of 87.5 and 207%,
respectively. Immobilized BGL exhibited higher pH and
temperature stability compared to free form. Thermal sta bility of hybrid improved up to 26-fold at 60 C. After 10
cycles of reuse, immobilized enzyme retained 93.8% of
residual activity. These results suggested that metal ions
played a significant role in the enzyme immobilization as a
protein-inorganic hybrid. Overall, this strategy can be
potentially applied to enhance the properties of enzymes
though effective encapsulation for the broad biotechnological.
