Abstract:
Some types of fungi are known to have the ability to produce Indole Acetic Acid (IAA). Fungi can be
isolated from the rhizosphere and tissues of various plants, including from the rhizosphere and the
root "Galam" (Melaleuca cajuputi Powell.), which grow predominantly in peatlands. Therefore, the
purposes of this study were: (a) to isolate and measure the potential of fungi from endophytic and
rhizospheric of “Galam”(M. cajuputi) as a producer of IAA hormone, (b) determine the types of fungal
interaction that occur and their potential to increase the total IAA hormone produced. This research
begins with isolation, purification, isolate screening, analysis of IAA hormone production, data
analysis, seed germination test and isolates identification. The result showed that the concentration
of IAA produced by Penicillium sp. IRZ15 was 5.86 ± 0.47 μg.mL-1 to 8.46 ± 0.26 μg.mL-1 and
Syncephalastrum sp. AG15 is 4.77 ± 0.44 μg.mL-1 to 8.77 ± 0.25 μg.mL-1. Meanwhile, the combination
of rhizospheric fungi Penicillium sp. IRZ15 and endophytic fungi Syncephalastrum sp. AG15 does not
produce significantly different IAA concentrations (6.42 ± 0.34 μg.mL-1 to 9.19 ± 0.50 μg.mL-1 )
compared to fungi used alone without combinations.
Keywords: endophytic fungi, Galam, Melaleuca cajuputi Powell., Indole Acetic Acid, peatland,
rhizospheric fungi
