Abstract:
Background: The process of wound healing is influenced by various factors such as age, hormones, and wound
care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is
traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant
that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate
the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male
Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty
male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa
leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method
with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The
wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each
group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and
observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3
fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five
experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and
positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of
fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with
the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on
the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a
significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.
KEYWORDS: Wound Healing, Rhodomyrtus tomentosa, Wound Length, Fibroblast.
