Abstract:
Mursyidin DH, Nazari YA, Badruzsaufari, Masmitra MRD. 2021. DNA barcoding of the tidal swamp rice (Oryza sativa)
landraces from South Kalimantan, Indonesia. Biodiversitas 22: 1593-1599. The tidal swamp rice (Oryza sativa L.) landraces of the
South Kalimantan, Indonesia, has been known for hundred years ago with a better adaptation to the local conditions, such as acidity,
salinity, and metals contamination. However, the genetic insight of these landraces has not fully understood. Here, the rbcL region of
tidal swamp rice from this region was successfully sequenced, aligned, analyzed, and deposited into the GenBank with accession
numbers of MT818188–MT818201. The multiple alignments of these sequences showing a barcoding motif with eight mutation or
polymorphic sites with both substitutions (transition-transversion) and indels (insertion-deletion). Based on this marker, the tidal swamp
rice has a low genetic diversity, only 0.086. However, the UPGMA and maximum likelihood (ML) analyses revealed that this
germplasm was grouped into five and twoclusters or clades, respectively. In this case, Bayar Putih is closely related to Siam Panangah
and farthest from Lemo. This information might help to develop conservation and breeding programs of the tidal swamp rice landraces
in the future.
Keywords: Breeding program, DNA barcoding, genetic diversity, rice, tidal swamp area
