The Quality of Boer Goat Semen Preserved with Sugar Palm Juice

RIZAL, MUHAMMAD; RIYADHI, MUHAMMAD; SULAIMAN, ABRANI

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Universitas Lambung Mangkurat
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The Quality of Boer Goat Semen Preserved with Sugar Palm Juice

RIZAL, MUHAMMAD; RIYADHI, MUHAMMAD; SULAIMAN, ABRANI

URI: https://repo-dosen.ulm.ac.id//handle/123456789/19057

Date: 2018-05

Abstract:

The objective of this study was to examine the effect of seminal plasma on viability of Boer goat spermatozoa and effectiveness of sugar palm juice as an alternative extender during preservation at 5ºC. Semen of two Boer goats were collected using an artificial vagina. Fresh semen were evaluated and divided in equal volume into four tubes. Semen in the first and second tubes diluted with 80% sugar palm juice + 20% egg yolk (P1) and Andromed (P2), respectively. Semen in the third and fourth tubes were centrifuged with 3,000 RPM for 20 minutes, and the supernatant removed. diluted with 80% sugar palm juice + 20% egg yolk (P3) and Andromed (P4), respectively. Dilutedsemen were preserved in refrigerator at 5oC, and quality of the spermatozoa including motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) were evaluated every day for four days. Results of this study showed that at day-2 preservation, mean percentages of MS, LS, and IPM for P2 (72, 83.4, and 83.4%), P3 (72, 82.6, and 82.2%), and P4 (72, 83, 83.8%) were significantly (P<0.05) higher than P1 (3, 24.8, and 25.2%). At day-3 preservation, mean percentages of MS, LS, and IPM for P2 (57, 65.6, and 69.6%) was significantly (P<0.05) higher than P3 (21, 34.8, and 31.8%), P4 (22, 33.6, and 31.2%), and P1 (0, 0, and 0%). In conclusion, semen of Boer goat to be preserved with extender containing egg yolk should be removed seminal plasma. Sugar palm juice containing egg yolk could be used as an extender for Boer goat semen, but should be applied in the AI program immediately after the semen is diluted.

Description:

Boer goat is a type of meat goats with a good body conformation, adaptable to changes in ambient temperature, and more resistant to disease (Malan, 2000). The Boer goat is a superior goat imported from South Africa. One of the objectives of importing Boer goats is to improve the performance of Indonesian local goats, such as Kacang goats. According to Inounu et al. (2002) one way to increase livestock productivity is to include superior males from foreign breeds to be crossbred with local females. The application of artificial insemination (AI) technology is a method that can be carried out to accelerate the population growth and to improve the genetic quality of goats. Through the integration of AI into semen processing technology, the reproductive potential of superior males can be optimized for mating with females. This objective can be achieved if the semen is diluted with certain diluents, meeting the quality requirements such as being energy source, buffer capacity, non-toxic, preventing damage to spermatozoa, cheap, and easy to obtain. According to Rizal et al. (2003) one ejaculation of male sheep can be used to mated about 35 females when using an AI program by using frozen semen packed in a mini straw; compared to the natural mating where one ejaculation can only be used to mated one female. One of the main problems in handling goat semen is the presence of enzyme in seminal plasma. The enzyme is synthesized by the bulbouretralis glands (Cowper glands) which, when interacting with egg yolk or milk, will cause coagulation of semen (Ritar and Salamon, 1982; Leboeuf et al., 1998; Leboeuf et al., 2000). The enzyme is identified as phospholipase A that can hydrolyzed yolk lecithin into fatty acids and lysolecithin. These fatty acids and lysolecithin from the hydrolysis are toxic to goat spermatozoa. Meanwhile, in the process of semen processing, the egg yolk and milk are commonly used as the components of the semen extender. The lecithin contained in the egg yolk is the main reason for its use as the component of semen extender. Lecithin is needed as the protector of spermatozoa plasma membrane from cold shock when semen is stored at low temperatures (Quinn et al., 1980; Watson, 1981). This problem can be solved by two alternative methods. The first method: if one of the components of semen extender is egg yolk, so the seminal plasma should be removed by centrifugation. According to Ustuner et al. (2009) and Naing et al. (2011) the separation of seminal plasma increases the spermatozoa motility of Saanen and Boer goats after thawing. The second method: if seminal plasma is not removed, it is better to use a semen extender that does not contain egg yolk. Andromed is one of the soybean-based commercial semen extender that does not contain egg yolk, so it can be used for an alternative extender in preservation of goat semen. The utilization of natural components such as sugar palm juice as the component of semen extender can be an alternative to synthetic chemical compounds which are expensive. Sugar palm juice contains 9.16% water, 84.31% sucrose, 0.11% fat, 2.28% protein, 3.66% total mineral, 1.35% calcium, and 1.37% phosphorus (P2O5) (BPTP Banten, 2005). Sugar palm juice also contains vitamin A and C and has a pH of 6-7. This fact about the content of chemical compounds is the basis why sugar palm juice can be utilized as one of the alternative semen extender. Sugar palm juice as an alternative extender has been reported to work well in the preservation and application of AI in swamp buffaloes (Rizal and Riyadhi, 2016). The objectives of this study was to examine the effect of seminal plasma on viability of Boer goat spermatozoa and the effectiveness of sugar palm juice as an alternative extender during preservation at 5oC.

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