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EPIDIDYMAL SPERMATOZOA QUALITY OF ETAWA CROSSBREED GOAT IN TRIS EXTENDER SUPPLEMENTED WITH VARIOUS LACTOSE CONCENTRATIONS

EPIDIDYMAL SPERMATOZOA QUALITY OF ETAWA CROSSBREED GOAT IN TRIS EXTENDER SUPPLEMENTED WITH VARIOUS LACTOSE CONCENTRATIONS

RIYADHI, MUHAMMAD; SETIAWAN, AGUS; HERDIS; RIZAL, MUHAMMAD
URI: https://repo-dosen.ulm.ac.id//handle/123456789/19041
Date: 2017-03

Abstract:

This research was conducted to investigate the effect of various concentrations of lactose supplementation in Tris extender for maintaining the quality of Etawa crossbreed goat epididymal spermatozoa stored at 3-5 C. Semen in the control group was diluted with a tris extender containing 20% egg yolk without lactose. Semen in the test groups was diluted with a tris extender containing 20% egg yolk and added with 0.3% (0.3 g per 100 mL extender) and 0.6% lactose for group TL1 and TL2, respectively. Parameters evaluated of the fresh epididymal spermatozoa were motility, concentration, percentage of live, and abnormality of spermatozoa, while for dilutedspermatozoa were motility and percentage of live spermatozoa. Spermatozoa observation was conducted until it reaches 40% motility. The results showed that the mean percentage of motility, live sperm, concentration, and abnormality of epididymal spermatozoa were 70%; 81%; 3,220x106 cells/mL; and 4.30%, respectively in all group. After dilution, the percentage of motility and live spermatozoa were also 70% and 81.00±1.58%, respectively in all groups. The decreasing of spermatozoa motility was observed on day 4 of storage, in which percentage of spermatozoa motility in control group (40.00±0.00%) was significantly lower (P<0.05) than those in TL1 (44.00±2.24%) and TL2 (45.00±0.00%) groups. Percentage of live spermatozoa in control (63.20±2.68%) was not significantly different (P>0.05) than TL1 (65.40±1.95%) and TL2 (65.60±1.95%). In conclusion, the supplementation of lactose into Tris extender could maintain the epididymal spermatozoa of Etawa crossbreed for 3 days of storage at 3-5 C.

Description:

The application of reproductive technologies for the population improvement of domestic animals has been developed through the application of artificial insemination (AI). Semen used for AI was usually collected using an artificial vagina (AV), but there is an alternative to obtain the spermatozoa from the cauda epididymis (Rizal et al., 2007). Axner et al. (1998) states that the spermatozoa obtained from the cauda epididymis have similar ability to the spermatozoa of ejaculated semen especially in motility and oocytes fertilization. Therefore, the use of cauda epididymis from abattoir waste can be used as an alternative of spermatozoa sources. Previous studies of spermatozoa collected from cauda epididymis have been reported in several animals such as in cows (Graham, 1994), deer (Soler et al., 2003), african buffalo (Herrick et al., 2004; Harshan et al., 2005; Herold et al., 2006), and Garut sheep (Rizal et al., 2004). Cauda epididymis is a temporary spermatozoa reservoir prior to ejaculation. Therefore, spermatozoa obtained from the cauda epididymis need to be proceed appropriately in order to survive for a certain duration and have an optimum fertility (Solihati et al., 2008). The processing needed to maintain the viability and fertility rates are through the addition of diluent. Various diluent could be used to dilute the spermatozoa, but it must contain substances required by sperm cells. According to Toelihere (1993), a diluent should be able to provide nutrients for spermatozoa, protecting the spermatozoa from cold shock, providing a buffer to prevent the pH changes, maintaining the osmotic pressure and electrolyte balance, preventing the growth of bacteria, and increasing the volume of insemination. One of the substances that can maintain the quality of semen is carbohydrate particularly sugars group (saccharides), either monosaccharide, disaccharide or oligosaccharide. This is due to the ability of carbohydrates as a resource of nutrients, as well as an extracellular cryoprotectant that able to protect the cell plasma membrane from damages (Supriatna and Pasaribu, 1992). The use of sugar for maintaining the quality of spermatozoa during the preservation and the cryopreservation process in livestock has been widely reported by several researchers, such as the use of glucose in the sheep frozen semen (Molinia et al., 1993), trehalose in pampinta sheep frozen semen (Aisen et al., 2000; Aisen et al., 2002), sucrose and trehalose in cattle frozen semen (Woelders et al., 1997), lactose in goat frozen semen (Singh et al., 1995), and Garut sheep liquid semen (Rizal, 2006), and dextrose, trehalose, raffinose, and sucrose in Garut sheep frozen semen (Rizal et al., 2006). In this research, lactose derived from the disaccharide group which function as an energy source, a water removal from the cells to reduce the ability of water to form ice crystals, and as an osmotic buffer to prevent the cell from swelling and to stabilize the cell membrane (Tambing et al., 2003) was used. Based on the previous studies, the addition of lactose in tris-egg yolk as extender for spermatozoa obtained from cauda epididymis of etawa crossbreed (PE) goat was expected can be an energy source for the spermatozoa and reduce the destruction to the plasma membrane of spermatozoa due to lipid peroxidation. Therefore, it will be able to produce a qualified liquid semen of PE goats.

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