Repo Dosen ULM

The fertility of South Kalimantan buffalo spermatozoa after cold preservation and cryopreservation

The fertility of South Kalimantan buffalo spermatozoa after cold preservation and cryopreservation

RIZAL, MUHAMMAD; RIYADHI, MUHAMMAD; ANDI SYARIFUDDIN, NURSYAM; THAHIR, MUHAMMAD
URI: https://repo-dosen.ulm.ac.id//handle/123456789/19038
Date: 2020-11-05

Abstract:

Sugar palm juice can be an alternative extender of buffalo semen because it contains nutrients needed by spermatozoa during preservation. The objective of this study was to examine the fertility of South Kalimantan buffalo spermatozoa after the preservation and cryopreservation process. Semen was collected by the artificial vagina. Fresh semen was divided into two tubes in equal volume and then diluted with sugar palm juice extender with a different composition. Semen in the first tube was diluted with 80% sugar palm juice + 20% egg yolk, and then stored in a refrigerator at 5ºC (chilled-semen) for three days. Semen in the second tube was diluted with 73% sugar palm juice + 20% egg yolk + 7% glycerol. Diluted-semen was loaded in a mini straw and then stored in a liquid nitrogen container (-196oC) for seven days. A total of 22 adult female buffaloes aged 3–5 years were selected as acceptors and estrous synchronization by PGF2α. Thirteen females were inseminated with chilledsemen, and nine females were inseminated with frozen semen The results of this study showed that motility of spermatozoa after preservation for three days was an average of 45.83% and 38.33% for frozen-thawed semen. The percentage of pregnancy and birth for chilled-semen was 69.23% and 61.54%, higher than for frozen-thawed semen (66.67% and 55.55%), but there was no significant difference between treatment. It can be concluded that sugar palm juice is suitable as an alternative extender for South Kalimantan buffalo semen because it is able to maintain the quality and fertility of spermatozoa after preservation and cryopreservation.

Description:

Swamp buffalo (Bubalus bubalis) in South Kalimantan Province through the Minister of Agriculture's decree in 2012 has been designated as a separate family and named the South Kalimantan buffalo. The decree also emphasized that the South Kalimantan buffalo is a wealth of genetic resources of Indonesian local livestock that must be protected and preserved. One effective form of conserving buffaloes in South Kalimantan is to increase the population, so as to improve the welfare of buffalo breeders. The application of artificial insemination (AI) technology is one alternative method to accelerate population increase and improve buffalo genetic quality (Morrell, 2006). Preservation and cryopreservation of semen are an integral and inseparable part of AI technology (Sansone et al., 2000), and it is an essential method for conservation of germ cells and is suitable for the proper genetic management (Singh et al., 2013). Semen cryopreservation is an effective method in the preservation of superior male genetic resources directly related to AI technology so that its application will enhance the genetic quality improvement of livestock (Martinez et al., 2007). According to Hafez and Hafez (2000), the main purpose of processing semen is to increase the capacity of semen to serve more female. To achieve this goal, semen is diluted with certain extenders, which meet conditions such as energy source, buffer, non-toxic, prevent damage to spermatozoa, do not reduce spermatozoa fertility, do not impede the evaluation of spermatozoa after dilution, inexpensive, and easily obtained. Extracts of natural ingredients from vegetables and fruits and their seeds contain an important compounds for health, so it is able to maintain life (Aviram et al., 2000; Aviram et al., 2004). These natural extracts and infusions are used in semen extenders for preserving animal sperm (Sansone et al., 2000). Some natural ingredients commonly used as semen extender contains strong antioxidant compounds, thus protecting spermatozoa from oxidative damage during the cryopreservation process (Seeram et al., 2005; Tezcan et al., 2009). Utilization of natural materials such as coconut water in semen cryopreservation has been reported in buck (Daramola et al., 2016) and cattle (El-Sheshtawy, 2017; Tarig et al., 2017), with good results. Sugar palm juice can be an alternative extender of semen because it contains nutrients needed by spermatozoa during preservation (Rizal and Riyadhi, 2016), but do not have protective compounds of spermatozoa during the process of semen preservation at 5ºC and cryopreservation at -196ºC. In the process of preservation and cryopreservation of semen, extender must be added a special compound in the form of cryoprotectant which functions to protect spermatozoa from damage (Andrabi et al., 2008). Egg yolk is one component that is commonly used in the preservation and cryopreservation of semen. Egg yolk lipoprotein, cholesterol, and lecithin are the function to maintain the integrity of the plasma membrane lipoprotein of spermatozoa during preservation at low temperatures (Kumar et al., 1992). Specifically, in the cryopreservation process of semen, other than egg yolk, extender should also be added to intracellular cryoprotectant compounds such as glycerol which functions to minimize cell damage during freezing (Leboeuf et al., 2000). Through this treatment, it is expected that the quality of spermatozoa during preservation and cryopreservation of semen can be maintained, and does not reduce fertility when applied in AI technology. The objective of this study was to examine the fertility of South Kalimantan buffalo spermatozoa after the preservation and cryopreservation process.

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