Abstract:
Aim: Intra-abdominal adhesion (IAA) is the most significant complication after surgery. This study explores the active compound
from Eleutherine americana Merr. as a regulator of mitochondrial calcium homeostasis in IAA. This study aimed to evaluate
computationally the potential of E. americana Merr. extract as a mitochondrial calcium activator in IAA cases. Materials and
Methods: The sample was 14 active compounds from E. americana Merr. extract that was collected from the PubChem database.
Prediction as a calcium channel activator was conducted using Prediction of Activity Spectra for Substances SERVER. Human
intestinal absorption (HIA) and lethal dose 50 (LD50) were evaluated using SERVER. Protein target and pathway were evaluated
using STITCH, and computational analysis was done using Cytoscape. Results: The results showed that 10 bioactives from E.
americana Merr. extract have potential as calcium activators (Pa >0.3). The active compound of E. americana Merr. can be easily
absorbed in the intestine based on its HIA+ score (HIA >0.9). Conclusion: The application of this extract was possible due to
LD50 evaluation that showed that it was less toxic (LD50 <3 mol/kg-BW). E. americana Merr. extract regulates mitochondrial
calcium homeostasis, by modulation of two types of the mitochondrial calcium channels, VDAC1 and MARCK (mRyR).
KEY WORDS: Calcium activator, Eleutherine americana Merr., MARCK, VDAC1
