Abstract:
Basal plate rot is a major disease on shallot caused by Fusarium oxysporum. Endophytic fungi is promising to use
as antagonist agent to the pathogen. Endophyte is microbes that are living in plant cells and have an asymptomatic
characteristic. Nowadays, fungal endophyte is believed to produce antimicrobial substances similar with their plant
host's natural product. Jatropha curcas is one of the plants containing secondary metabolites that have antifungal
activities. The research aimed to study the ability of endophyte from Jatropha curcas to inhibit the growth of Fusarium
oxysporum. The dual culture method was used in this research and the data were analyzed by SPSS software. This
antagonism test was conducted by 9 isolates endophyte and each plate consisted of 3 replicates. The result revealed
endophyte fungal obtaining 9 isolates with the radial growth of 4,5 cm/2 days. Endophytes of Jatropha curcas L. were
able to inhibit the growth of Fusarium oxysporum C2. The percentage of inhibition of Fusarium oxysporum causing
of root blight diseases was controlled by up 38.27 - 74.48%. The highest percentage of inhibition is gained by B4b
and the lowest of it is A2b. Our observations showed that each endophyte has a consistent linear trend. B4b still
leaded as the highest strength to inhibit the growth of pathogen on the monitoring of 3, 5, and 7 days. Moreover, the
ability of fungi endophyte from Jatropha curcas as antagonist agent to Fusarium oxysporum needs to be further
examined by the in vivo method.
Keywords: Biological control; Percentage of inhibition; Root blight; Inhibition mechanism
