A common problem often faced by healthcare service management is the difficulty in finding qualified nurses. Training is one of the characteristics of an organization that has a positive impact on increasing professionalism and the work performance of nurses at the hospital. Improving supervision is a method to monitor the quality of services by hospital supervisors. This study attempts to determine the effect of clinical supervision training as one of the organizational characteristics on increasing the work performance of nurses at the hospital. The quasi-experimental study design was used in this study with 68 nurses who were working in a hospital in Banjarmasin. The observation was carried out before and after training. The dependent variable in this study is clinical supervision training, and the independent variable is work performance. Univariate, and bivariate were used in data analysis. There was an effect of clinical supervision training as one of the organizational characteristics on increasing the work performance of nurses at the hospital (p=0.00). Clinical supervision training increased work performance on clinical supervision abilities. Nurse training improved the quality of healthcare services, especially nursing care. Keywords: clinical supervision, nurse, work performance