People nowadays are identified to have negative consumption habits, due to their preference to consume high-leveled calorie fast food and unhealthy snacks. This habit results in an increase of metabolic risk factors that affect general health. In response to the case, the innovation of healthy snacks is sought after to remedy the situation. This study proposes the production of healthy snacks in the form of glucose friendly biscuits. The study utilizes sutchi catfish (Pangasius hypophthalmus) cracker biscuit which contains EPA and DHA as a healthy snack alternative in order to reduce the glucose level and increase the insulin sensitivity. The study aims to determine human blood glucose levels after consuming sutchi catfish (Pangasius hypophthalmus) cracker biscuits. The methods were included in phase 1 clinical trials where 30 people were divided into 3 treatments: negative control (placebo was given), positive control (white bread was given) and sutchi catfish cracker biscuits as test foods with 10 times replications. The result shows a tangible result in terms of reducing the level of glucose level within human blood, 2 hours after the consumption of sutchi catfish filrate-based cracker biscuits. The percentage reduction in the treatment of sutchi catfish cracker biscuits was 22.64% within the normal range of general blood glucose levels. In accordance with the results, it can be concluded that sutchi catfish filtrate-based biscuit crackers can be considered as nutraceutical or healthy diabetic cookies. It is healthy and safe (glucose friendly biscuits) to be consumed by most people, particularly those who suffer from diabetes me