Glucose is important carbohydrate which is more absorbed into the bloodstream as glucose and other sugar is changed into glucose in the liver. Blood glucose level is closely related to diabetes mellitus. Diabetes mellitus is a disease that arises in a person which is indicated by the presence of blood glucose level exceeding normal (hyperglycemia) due to the deficiency of the insulin hormone in the body. The design of this research was using RAL with 24 male rats that divided into 6 treatments which were normal with the giving of Na-CMC 0.5% 1mL/200 gram BB, negative with the giving of Na-CMC 0.5% 1mL/200 gram BB, positive with the giving of glibenclamide of 0.45 mg/kg BB and 3 other treatments by giving catfish biscuit (Formula A, formula B and formula C) with 4 replications. All treatments were injected with monohydrate alloxan at a dose of 150 mg/kg BW, except for the normal treatment. The results showed that the formulation of catfish biscuit can lower the blood glucose level of white rats. The composition of biscuit with formula C has the highest decreasing percentage which was 80.20% to the blood glucose level of white rats.