Blood leukocyte differentiation is one parameter to determine the health status of the animals, because of the differentiation of blood leukocytes is a component that has an important function as the body's first defense when disease or foreign substances into the body. Katuk is a medicinal plant that has several active substances that serve to health. This study aims to determine the leukocyte differentiation from sheep given the leaf extract fraction katuk. Complete randomized design (CRD) was used with 4 replications and 4 treatments fraction katuk leaf extract. The treatments include: control, Ethanol crude extract (1500 mg / day of extract ETOH), delipidasi fraction (1230 mg / day FDL) and hexane fraction (270mg / day FH). Fractions katuk leaf extract given to sheep in capsule form that is 2 times a day for 2 months. Leukocyte differentiation calculation performed after 2 months of treatment. The results show that the value of leukocyte differentiation sheep after administration leaf extract fraction katuk namely lymphocytes, neutrophils, monocytes, eosinophil and the sequence is (41.5-46)%, (47.7-54,7)%, ( 1.5-2.7)%, (2.2-6)%. Leukocyte differentiation value in this research is still in the normal range sheep. This shows that the treatment of fraction katuk leaf extract does not interfere with physiological health status of sheep.