Catfish oil extract contains albumin and w-3 fatty acids which play a role in wound healing. The purpose of this study was to determine the potential of catfish oil extract (Pangasius hypophthalmus) in healing white rat wounds on the area of the wound and the percentage of wound healing. Rats were grouped into 7 treatments, namely positive control with administration of betadin ointment, negative control was not given and 4 other treatments with administration of catfish oil extract (A, B, C, D), treatment E was given tween 20. White rats were injured in the area back with a length of 2 cm and a depth of 2 mm. Rat treatment is done for 7 days topicallyThe results of analysis in this study showed that catfish oil extract (Pangasius hypophthalmus) has the potential to heal the wounds of white rats in the presence of decreased wound area of about 0.62 mm - 0.84 mm and the percentage of wound healing around 79.00% - 84 , 45%.