Diabetes mellitus is one of degenerative diseases, which is a disease that caused by a decreasing in function or structure of tissues or organs progressively from time to time, and it is caused due to age or lifestyle choices. Moreover, it can be caused because the increasing of blood sugar (glucose) level that caused by the insulin deficiency both absolute and relative, so that the regulation of blood glucose level becomes chaotic. Hyperglycemia can be treated with diabetes mellitus therapy which is given to the patients with a target to reduce the blood glucose level to be normal or it is called hypoglycemic . This study aims to determine the hypoglycemic activities of catfish biscuits on the blood glucose level of white rats. The design of this study used RAL with 24 male mice divided into 6 treatments, namely normal with the giving of 0.5% Na- CMC 1mL/200 gram BB, negative with the giving of 0.5% Na-CMC 1mL/200 gram BB, positive with the giving of 0.45 mg/kg BB glibenclamide and 3 other treatments by giving catfish biscuits (Formula A, formula B and formula C) with 4 times replication. All treatments were injected with monohydrate alloxan with a dose of 150 mg/kg BB, except for the normal treatment. The result showed that the hypoglycemic activities of catfish biscuits can reduce the blood glucose level in white rats with the hypoglycemic power which is 42.47%.