Sasmita R, Mabrur, Rahmy USA, Badruzsaufari. 2019. Genetic diversityand phylogenetic relationship amongAnabantoidei fish(Anabantiformes) inSouth Kalimantan, Indonesia based onSDS-PAGE analysis.Biodiversitas 20:2519-2527.Protein profile is a molecular marker for diversity andphylogenetic analysis of germplasm, includingAnabantoideifish which is abundant in freshwaterecosystem of South Kalimantan. The aim of this research wasto analyze genetic diversity and construct phylogenetic relationship amongAnabantoidei fish based on SDS PAGE analysis. Proteinwas extracted from muscle tissue of six species of Anabantoidei fish andprecipitated using Ammonium sulfate salt. Soluble proteincontentwasdetermined using Bradford assay and thenseparated based on SDS-PAGEmethod. Genetic diversity and phylogenetic relationshipwere constructed using PASTsoftware based on UPGMA method.The results showed that the soluble protein of fish muscle can be precipitated optimally in different ammonium sulfate concentration. Based on SDS-PAGE analysis, 59 different protein bands have beenseparated from gels with molecular weight ranging from 28.15 to 181.61 kDa. On the protein level, the Anabantoidei fish showed high genetic polymorphism(greater than 90%) with 3, 2 and 5 monomorphic bands on non-precipitated, AS-precipitated and combination between non-and AS-precipitated proteins,respectively.The phylogenetic reconstruction also exhibited that the Anabantoidei fish has the unique phylogenetic trees, especially for the combined protein datasets. This information would be useful for freshwater fish conservation and breeding programs. Keywords: Anabantoidei, Bradford, diversity, protein profile,SDS-PAGE