Abstract. This research was conducted to study the effects of zeolite in reducing aflatoxin residues and liver histopathology of laying duck. Sixty-four of Indonesian local laying duck (Anas platyrinchos Borneo) were randomly allocated to 2 levels of aflatoxin B1 (AFB1) (low: 30 ppb; and high: 70 ppb) and 2 levels of zeolite inclusions (0 and 2%). The trial was conducted for 28 days and at the end of treatment, the ducks were sacrificed. Meat, liver, and egg samples were collected for AFB1 and aflatoxin M1 (AFM1) determinations. AFB1 and AFM1 concentrations were determined using ELISA analysis. Data were analyzed by analysis of variance using the general linear model of SPSS software. Liver samples were also analyzed for the histopathological study. Results showed that levels of AFB1 significantly (P<0.05) increase AFB1 concentration in the liver and egg. Zeolite inclusion did not significantly (P>0.05) reduce AFB1 and AFM1 concentrations in meat, liver, and egg. Examination of liver samples indicated moderate and severe liver pathology in the diet without zeolite. Therefore, it was concluded that zeolite inclusion in the high AFB1 contaminated diet does not reduce aflatoxins residue in the tissues but could prevent liver alteration of laying duck.