This study provides meaningfully scientific information on the attempt to accelerate the gonad rematuration of snakehead (Channa striata) to support the quality seed production for both commercial and restocking purposes in South Kalimantan, Indonesia. A total of 48 snakehead fish broods (296-312 mm total length and 234-298 g weight) were subjected to different treatment levels using oodev dose of 0.5 ml kg-1 fish weight to determine the best frequency and time period for gonad rematuration after spawning. Treatment-A: 3 times/9 days, B: 2 times/6 days, C: 1 time/3 days, and D: no oodev injection (control). The 12 hapas were used comprising 4 individuals per hapa (0.40.41 m) under the controlled condition. After all, ten snakehead brood samples were dissected to find out the gonad maturity level of fish. The treatment-A showed the best performance in term of mean fecundity (19600±450.00 granules/individuals), egg diameter (1.10±0.15 mm), and gonad somatic index/GSI (3.41±0.90 %) among other treatments. Dealing with hepatosomatic index (HSI), the treatment-C was significantly higher than treatment-A and D, but not differ from treatment-B. The mean HSI was varied from 0.89±0.03 % to 1.53±0.23 %. Temperature, pH and DO during sampling period are considered comfortable for snakehead fish broods. Keywords— Channastriata, gonad rematuration, fecundity, egg diameter, GSI, HSI