Abstract. Herbal medicine in pharmaceutical therapy has widely studied and developed. However, Aquilaria microcarpa as one of plants existing in South Kalimantan, has not been studied for its benefit yet. Aquilaria microcarpa leaves extract has been proved could lowered mice blood sugar level, probably because of its flavonoid content. Flavonoid were known has antioxidant activity. Extract and fraction were difficult to apply, therefore it is needed to make them to a dosage form. This study was aimed to determine antioxidant activity of gel consisting ethanolic extract and ethyl acetate fraction of Aquilaria microcarpa leaves. Antioxidant activity were evaluated for IC50 value using DPPH (2,2-diphenyl-1-picrylhydrazyle) methode. Extract were obtained from ultrasonicatedmaceration in ethanol 96% solvent. Extract were processed further in nhexane and ethyl acetate, where latter fraction were used. Each extract and fraction then made to gel, using HPMC as gel-base (1-2%). Result showed IC50 value for gel extract were 26,39 – 28,94 ppm and for gel fraction were 22,22 – 23,05 ppm. Both has highly active antioxidant activity. Keywords: Aquilaria microcarpa leaves, ethanolic extract, ethyl acetate fraction, antioxidant activity, gel