Wastewater is liquid or filth containing hazardous materials that can endanger human life and other living beings and is also destructive the environment. This research aim to analyze the differences in physical parameters (Temperature and TSS), chemical (pH, BOD, NO3-N, TF-P, oil and fat) and biology (E. coli) before and after treatment. The research design is observational analytic throught cross-sectional approach. The object used is domestic wastewater before and after treatment in WWTP Lambung Mangkurat Banjarmasin. Data collected from WWTP report on 2015. This research use paired t-test and Wilcoxon test. The results in WWTP Lambung Mangkurat showed that average value before and after treatment in TSS (p = 0.003), pH (p = 0.002), BOD (p = 0.008), TF-P (p = 0.003), E. coli (p = 0.003), temperature (p = 0.059), NO3-N (p = 0.424), oil and fat (p = 0.086). There is a difference before and after treatment in TSS, pH, BOD, TF-P and E. coli. Whereas there was no difference in temperature, NO3-N, oil and fat.