The incidence of HIV/AIDS is a global problem. The highest cases occurred in the age group of 20-49 years indicated they are already HIV positive when adolescence (15-25 years). The cause of the high incidence of HIV/AIDS among adolescents is influenced by many things including their lack of knowledge about HIV/AIDS. This study aim§ to detertnine the effect of counseling and sexual education toward knowledge and attitude of Banjarbaru Midwife Academy Student. This study uses a quantitative method with pre-experimental research design. Data collected through questionnaire from Batijatbaru Midwife Academy Student. The results show the number of students who have good knowledge increased after being given counseling, froin 3 5% to 70%. In addition, the number of students who have a good attitude after being given counseling increased, from 87.5% to 100%. The conclusion of this study is counseling about HIV/AIDS can affect Banjarbaru Midwife Academy Student knowledge of 4.206 times and attitudes by 4.206 times.