"There are several plants in Kalimantan that local people use to treat malaria such as Hydrophylla spinosa leaves, Ampelocissus rubiginosa tubers, Uraria crinita roots, and hati tanah (Angiopteris evecta) tubers. World annual cases of malaria and the increasingly wide plasmodial resistance to antimalarial medicines must be overcome by finding new antimalarial medicines. The study aims at finding out in vitro antiplasmodial activity of A. evecta tubers in the culture P. falciparum train FCR3. A kilogram simplisia is extracted using ethanol and the result is 250.5 g dried extract. It means that ethanol extract yield is 25%. Fractionation is carried out by triturating the ethanol extract and it gives 3 fractions, which are FA 5.15%, FB 36.5%, and FC 52.25% of 20 g ethanol extract (EE). The chromatogram profile of the FA, FB, and FC contents is obtained using KLT of chloroform-methanol moving phase (9:0.5 v/v). The in vitro antiplasmodial activity of each of the fractions in the culture P. falciparum FCR3 is tested in the incubation period of 72 hours. The results of the in vitro antiplasmodial activity test of the FA, FB, and FC in triplets are mean parasitemia percentage, inhibition, and IC50 value. The results of the in vitro antiplasmodial activity test in triplets of the FA, FB, and FC of the A. evecta tubers are mean IC50 values of 37.93 ± 1.19 μg/mL, 2.86 ± 0.27μg/mL, and > 250 μg/mL, respectively. The in vitro antiplasmodial activity of the FB is categorized into very active or very potential. Keywords: Angiopteris evecta, antiplasmodial in vitro, antimalarial, bioassay guided"