The objectives of the research is : (1) Search is apllied technology of biculture of sea weed and sea cucumber to increase of productivity and oplimatization of marine culture; (2) Know of culture method of sea weed (Eucheuma cottonii) is very good for its growth and (3) Know of optimalize density of sea cucumber (Holothuria scabra) is very good for its growth. Location of the research is Teluk Tamiyang village, Kotabaru district, South of Kalimantan Province. The research was carried out in 14 weeks (3.5 moths), from Juni to September 2006. The research applied experemental design of Completely Random Design (CRD) 2 x 2 and 3 replications. First factor is aquaculture methods (M) with 2 degree is M1 = Long line method and M2 = Off bottom method, and second factor is sea cucumber density (P) with 2 degree is P1 = 10 ind/m2 and P2 = 20 ind/m2. The research results was : (1) daily growth of sea weed was 17.53 – 19.26 %, which was quickly growth catagori; (2) weight relative growth of sea cucumber was 108.15 – 118.25 % and survival rate was 89.0 – 95.3 %; (3) Analyzed of statistic showed aquaculture method factor was significant to growth of sea weed, which off bottom method than better long line method, but not significant to growth and survival rate of sea cucumber; (4) Density factor of sea cucumber was not significant to growth of sea weed and growth and survival rate of sea cucumber; (5) Biculture of sea weed and sea cucumber was not opposite, so than better monoculture and (6) Site location quality and water quality of Teluk Tamiyang was good and ideal to biculture of sea weed and sea cucumber.