IMPROVE SPAWNING QUALITY OF KISSING GOURAMI (HELOSTOMA TEMMINCKII) WITH COMMERCIAL FEED CONTAINING GLUTATHIONE AND VITAMIN E

Abstract

This research is needed to accelerate spawning by adding glutathione and vitamin E in the feed to produce quality fish seeds. The aim is to determine the effect of adding vitamin E to commercial feed containing glutathione on increasing gouramy spawning. All treatment was carried out for two months using a Completely Randomised Design (CRD) with 4 treatments and 3 replications. Treatment A (glutathione), B (glutathione and vitamin E 150 mg.kg–1), C (glutathione and vitamin E 300 mg.kg–1), and D (glutathione and vitamin E 450 mg.kg–1). 12 broodstock with male:female ratio of 1:1, weight range of 90-130 g obtained from nature. They fed as much as 5% of the fish body weight thrice daily. The results showed that the highest hatching rate was 99.67% in treatment D. The egg diameter in treatment C showed the best results (0.88 mm). The highest fecundity was 21.257 g in treatment D. The highest survival was 95.56% in treatment D. All feed treatments with a combination of glutathione and vitamin E gave a better response than the addition of glutathione alone. Polynomial regression analysis of the egg diameter parameter showed that the optimal dose of vitamin E was 282 mg.kg–1