Plagiasi : Effect ofNauclea subdita(Korth.) Steud.leaf extract on hematological and histopathological changes in liver and kidney of striped catfish infected byAeromonas hydrophila

Abstract

Aim:The present study was conducted to investigate the efficacy of different doses of Bangkal (Nauclea subdita) leaf extract on hematological and histological changes in kidney and liver of catfish (Pangasius hypophthalmus) infected by Aeromonas hydrophila. Materials and Methods:Catfish were experimentally infected withA. hydrophilaat a dose of 108cells/mLthrough intraperitoneal injection, and the hematological and histological changes in the kidney and liver of catfish against the pathogen were observed. Results:Not all concentrations ofN. subditacaused a toxic effect in striped catfish. The clinical symptoms of catfish after infection withA. hydrophilaand treatment withN. subditaleaf extract included morphological and behavioral changes. N. subditaleaf extract reduced mortality caused byA. hydrophila. Treatment withN. subditaleaf extract was effective in reducing the inflammation by decreasing the activity of neutrophils, monocytes, and lymphocytes. The Hb and Ht levels of catfish significantly decreased after exposure to 108cells/mL ofA. hydrophilaand increased significantly afterN. subdita treatment. Necrosis percentages in the kidney and liver also decrease afterN. subditatreatment. Conclusion:The results indicate thatN. subditaleaf extract stimulates the immunity and increases the resistance of catfish toA. hydrophila. N. subditaleaf extract may be used as a potential source for future drug development and food applications.