Korespondensi : Effects Of Jeruju Leaves Extract (Acanthus ilicifolius) On Sangkuriang Cat Fish (Clarias gariepinus) Infected With Aeromonas hydrophila : Clinical Symptoms and Differential Leukocytes

Abstract

This study aimed to determine the effect of ethanol extract of jeruju leaves on clinical symptoms and differential leukocytes of sangkuriang catfish infected with Aeromonas hydrophila. This research was conducted experimentally using a completely randomized design (CRD) consisting of five treatments and three replications. Sangkuriang catfish infected with A. hydrophila at a dose of 108 cells/mL via intraperitoneal injection. Then, the infected fish were treated with ethanol extract of jeruju leaves with concentrations A = 0 mg/L, B = 62.5 mg/L, C = 125 mg/L, D = 250 mg/L and K = control. Parameters observed were clinical symptoms and differential changes in leukocytes, including neutrophils, monocytes, lymphocytes, basophils, and eosinophils. The clinical symptoms of fish infected with A. hydrophila after being given jeruju leaves extract (according to the treatment concentration) recovered faster than those without the extract (K). The differential leukocytes showed the highest percentage of neutrophils and monocytes at 250 mg/L, basophils at 62.5 mg/L, the highest eosinophils at 125 mg/L, and the percentage of lymphocytes did not differ between treatments. It can conclude that the extract concentrations of 125 and 250 mg/L were effective in stimulating differential leukocytes of sangkuriang catfish, which was indicated by an increase in the percentage of neutrophils and monocytes