Abstract:
This research is needed to accelerate spawning by adding glutathione and vitamin
E in the feed to produce quality fish seeds. The aim is to determine the effect of adding
vitamin E to commercial feed containing glutathione on increasing gouramy spawning. All
treatment was carried out for two months using a Completely Randomised Design (CRD)
with 4 treatments 3 replications. Treatment A (Glutathione), B (Glutathione and Vitamin E
150 mg.kg–1), C (Glutathione and Vitamin E 300 mg.kg–1), and D (Glutathione and Vitamin
E 450 mg.kg–1). Twelve Brood stock with male: female ratio of 1:1, weight range of 90–130
g obtained from nature. They fed as much as 5% of the fish body weight thrice daily. The
results showed that the highest hatching rate was 99.67% in treatment D. The egg diameter
in treatment C showed the best results (0.88 mm). The highest fecundity was 21.257 g in
treatment D. The highest survival was 95.56% in treatment D. All feed treatments with
a combination of glutathione and vitamin E gave a better response than the addition of
glutathione alone. Polynomial regression analysis of the egg diameter parameter showed
that the optimal dose of vitamin E was 282 mg.kg–1
