Abstract:
Introduction: Caries is the most common oral disease found in
society. The prevalence of caries in South Kalimantan is as high as
46.9% in 2018. S.mutans is the causative microorganism in the initial
occurrence of caries. Strategy that can be used to prevent caries is by
adding nano-hydroxyapatite to the tooth paste. Nano-hydroxyapatite
can be obtained from Snakehead (Channa striata) fish bone.
Snakehead is a kind of fish that is abundant in Banjarmasin. This
study aimed to analyze antibacterial activity of the nano-
hydroxyapatite paste from snakehead (Ch anna striata ) fish bone
against S.mutans bacteria. Methods: The study consisted of 5
treatment groups: negative control (basic formula), positive control
(casein phosphopeptide-amorphous calcium phosphate or CPP-ACP)
and three treatment groups (nano-hydroxyapatite paste
concentration of 10, 20 and 30%. The paste was made in the
formulation of F1, F2, and F3. The antibacterial activity test by
measuring MIC and MBC were performed using dilution method.
Results: MIC of nano-hydroxyapatite paste was at a concentration of
10% with an average value of the difference absorbance of -0.468.
MIC values in the concentration of 10%, 15%, 20% positive and
negative control groups had a significant difference. MBC of nano-
hydroxyapatite paste was at concentration of 15%. Concentration of
10% and the negative control group showed a significant difference,
while concentration of 15% and 20% groups did not show a
significant difference. Conclusion: Nano-hydroxyapatite paste from
Snakehead fish bone has antibacterial activity in inhibiting and
eliminating mutated S.mutans bacteria. The most effective
concentration of antibacterial nano-hydroxyapatite paste to prevent
caries was 10%. At this concentration, nano-hydroxyapatite can
inhibit the growth of S.mutans without killing the bacteria.
