In Vitro Metabolite Profiling and Anti-Inflammatory Activities of Rhodomyrtus Tomentosa with Red Blood Cell Membrane Stabilization Methods.

Abstract

Background: Rhodomyrtus Tomentosa (Karamunting) is one of South Kalimantan's biodiversity. In previous research on asthma and coal dust exposure models, nebulization with an ethanol extract of R. tomentosa leaves has been shown to reduce inflammatory cells. This study aimed to investigate the anti-inflammatory activity on the stabilization of red blood cell membranes and to identify the chemical compounds present in extracts of R.tomentosa leaves. Methods: Anti-inflammatory effect of R. tomentosa leaves was evaluated by in vitro red blood cell membrane stabilization test. Nonsteroidal anti-inflammatory sodium diclofenac was used as the reference drug. UPLC-MS/MS determined the metabolite profiling ethanol extract of R.tomentosa leaves. Results: The results of inhibition of red blood cells cell membrane lysis showed statistically significant (p>0.05) in the n-hexane fraction (concentration 25 ppm), ethyl acetate fraction (concentration 50 ppm), and a fraction of water (concentration 50 ppm) with an inhibition level of 54.5%, 81.8%, 63.6% respectively. This result is not significantly different from the standard anti-inflammatory sodium diclofenac (90.09%). Oleanonic acid and ursonic acid were two similar metabolites in subfractions 1, 2, and 3, which may have anti-inflammatory properties. Conclusion: The n-hexane, ethyl acetate, and water fractions from the ethanolic extract of R. Tomentosa leaves can act as an anti-inflammatory by inhibiting red blood cell membrane lysis, depending on the concentration. The metabolites that may act as an anti-inflammatory are oleanonic acid and ursonic acid.