PENGARUH EKSTRAK DAUN KASTURI TERHADAP KADAR LIMFOSIT MENCIT JANTAN (Mus musculus) YANG DIPAPAR SINAR-X RADIOGRAFI PERIAPIKAL

Abstract

Background: Periapical radiographic X-ray exposure can cause blood cell damage, such as lymphocyte damage. Direct damage occurs when it attacks the DNA and RNA of cells. Indirect damage occurs through the formation of free radicals. One of the natural antioxidants that can be used is kasturi leaves. Kasturi leaf extract contains active ingredients such as flavonoids, phenols, alkaloids, terpenoids, and saponins that can inhibit free radical activity. Objective: To measure and analyze the effect of kasturi leaf extract on the increase in lymphocytes levels of male mice exposed to x-ray periapical radiographs. Methods: This study used a true experimental method with a post test only design with a control group design. The samples used were 20 male mice aged 3-4 months with 20-25 grams bodyweight. Mice were divided into 5 groups, each group consisted of 4 mice. Mice were given kasturi leaf extract and exposed to x-ray periapical radiographs. Mice lymphocytes levels were measured automatically using a hematology analyzer. Results: Lymphocyte levels in P1 group = 3,400 x 103/μL, P2 group = 5,725 x 103/μL, P3 group = 7,825 x 103/μL, P4 group = 9,750 x 103/μL, and P5 group = 5,375 x 103/μL. Conclusion: Differences in lymphocyte levels were influenced by antioxidants from kasturi leaf extract and large differences in the radiation dose received.