Prosiding - Thin Layer Chromatography Analysis Of Ethanol Extract Of Garcinia Forbesii King. And Its Cytotoxic Effect On MCF-7 Breast Cancer And HepG2 Liver Cancer Cell Lines

Abstract

Thin Layer Chromatography (TLC) analysis of ethanol extract of Garcinia forbesii King. and its cytotoxic effect on MCF-7 breast cancer and HepG2 liver cancer cell lines has been conducted. Parts of the plant examined were leaves, stem bark, wood, and pericarp. The extraction was processed by maceration method, while the determination of the compound was carried out using various color reagents on TLC plate. The cytotoxic effect was carried out using MTT assay. TLC analysis showed that ethanol extract of the leaves, stem bark, and wood contains polyphenols, flavonoids, and terpenoids; while ethanol extract of the pericarp contains alkaloids, flavonoids and terpenoids. MTT assay showed the IC50 values of ethanol extract of the leaves, stem bark, wood, and pericarp in MCF-7 cell line were 197.92 μg/mL, 437.21 μg/mL, 164.11 μg/mL, and 60.11 μg/mL, respectively; while the IC50 values in HepG2 cell line were 88.58 μg/mL, 1403.75 μg/mL, 111.71 μg/mL, and 47.10 μg/mL.