Bukti Koresponden, Isolasi Senyawa Terpenoid Dari Fraksi n-Heksana Daun Bilaran Tapah (Argyreia nervosa (Burm. F.) Asal Kalimantan Selatan

Abstract

One of the plants that is used empirically by people in South Kalimantan as a traditional medicine is bilaran tapah (Argyreia nervosa (Burm. F.). This plants that has purposed antiinflamation, antimicroba, antidiabetic, diuretic, and aphrodisiac. This research aims to isolate and explore the chemical compounds of n-hexana fraction of A. nervosa leaves. Extraction is conducted by using maceration method. Isolation is conducted by using Vacumn Liquid Chromatography and gravity chromatography column. Identification qualitative assay of compound used TLC test, UV-Vis and FTIR spectrophotometry. The extraction of 500 grams A. nervosa leaves rough powder with 96% ethanol extracts produces 16,92% of rendement. The fractionation of 30 grams ethanol extracts with n-hexane produces 23,60% of rendement. N-hexane fraction by using VLC with mobile phase n-hexane- ethyl acetate (25:1; 20:1; 15:1; 10:1; 9:1; 8:2; 7:3 ; and 6:4) v/v produces fractions A, B, C, D, E, F, G, and H. Fraction C is chosen to be isolated by using column chromatography with mobile phase n-hexane : ethyl acetate (20:1)v/v. TLC qualitative test with ammonia vapor, dragendorff, and Liebermann-Burchard reagent shows that C-4 isolates contains terpenoid compounds. Analysis of C-4 isolates by using UV-Vis spectrophotometry reach peak at λ 245 nm. Analysis FTIR spectra shows that the functional groups of C-4 isolates are -OH (3309.85 cm-1), -CH aliphatic (2939.52 cm -1 and 2870.08 cm-1), C = C (1635.64 cm- 1) and C- O (1033,85 cm-1).