Bukti Koresponden ,Aktivitas Penghambatan Polimerisasi Hem Dari Fraksi n-Heksana Dan Fraksi Larut Air Akar Manuran (Coptosapelta tomentosa Valeton ex K. Heyne) Asal Kotabaru Kalimantan Selatan

Abstract

Coptosapelta tomentosa’s root empirically used as an antimalarial by people in Sungai Buah, Kotabaru, South Borneo. C. tomentosa’s root contains a variety of chemical compounds, the presence of chemical compounds which are non-polar such as anthraquinone, flavonoids and terpenoids can provide antimalarial activity. Non-polar chemical compounds separated from other chemical compounds using the fractionation method with n-hexane and aquadestillata. Antiplasmodial activity in the n-hexane fraction and water soluble fraction of C. tomentosa’s root was tested in vitro by inhibition of heme polymerization methods and measured with IC50 values. Chloroquine diphosphate was used as a positive control. DMSO 10% and aquades were used as a negative control. A significant difference between the IC50 value of the test compound and the positive control was analized by independent sample t-test with 95% trust level. The results showed that the inhibition percentage of heme polymerization from n-hexane fraction of C. tomentosa’s root with a concentration of 5; 2.5; 1.25; 0.625; 0.3125 mg/mL consecutively is 98.54; 94.60; 92.41; 85.87; 63.69%. IC50 value of heme polymerization test compound is 0,15 ± 0.01 mg/mL, water soluble fraction is fraksi 1.970 ± 1.38 mg/mL, and chloroquine diphosphate is 4.02 ± 0.63 mg/mL. The results of analysis with independent sample t-test declare that there is no difference between both of them with a significance value is 0.073. It can be concluded that n-hexane fraction of C. tomentosa’s root has an equal potential as good as chloroquine to inhibit 50% heme polymerization.