Ekspresi Hypoxia-Inducible Factor-1α menginduksi Ekspresi Eritropoietin Intraseluler, dan Vascular Endothelial Growth Factor pada Penderita Kanker Payudara dengan Anemia

Abstract

Anemia is an independent prognostic factor for survival of cancer patients. Decrease oxygen capacity in blood can lead to hypoxia in tumors. Hypoxia in the tumor environment can activate the transcription factor hypoxia-inducible factor-1α (HIF-1α), which then transcribes many other genes involved in cell invasion, angiogenesis, anaerobic metabolism and cell cycle, such as erythropoietin (Epo) and vascular endothelial growth factor (VEGF). This study using 120 samples of breast cancer tissue (60 anemic and 60 non-anemic). Study using immunofluoresens double staining methods for HIF1α protein withVEGFandEpowiththeEpoR.Samplesweredividedinto2groups:anemic (Hb5,5-10,7)andnon-anemic(Hb11– 14,9). There was a significant difference of VEGF expression (p=0,013) between patients anemic (754,4 ± 316) and non- anemic (555,1 ± 276,9). There were no significant difference of HIF1α expression in both anemi and non-anemi breast cancerpatients.InanemicandnonanemicgrouptherewasanegativecorrelationbetweenHbandHIF1α (p=0.000,r=- 0,522)and positivecorrelationbetweenHIF1αandEPO(p=0,000;r=0,697),HIF1αandVEGF(p=0,000;r=0,644), Epoand VEGF (p=0,001; r=0,433). In anemic and non anemic cancer patients hipoxia condition has been occurred in tumour enviroment causingnosignificantdifferentofHIF1αexpression,howeverthereisastrongcorrellationbetweenHIFαand Epo as well as HIF α and VEGF.